自由基和其他活性种在许多心理和病理生理过程中发挥着重要作用。自由基一旦在细胞内产生，就会破坏多种细胞成分，包括蛋白质、脂质和DNA。然而，在较低浓度下，自由基可以作为细胞信号传导中的第二信使。
Enzo Life Sciences的ROS-ID® ROS/RNS detection kit可用荧光显微镜直接实时监测活细胞中活性氧与活性氮（ROS/RNS）的产生。该试剂盒中包含三种检测探针：NO检测试剂（红色，Ex/Em 650/670 nm），用于总ROS检测的氧化应激检测试剂（绿色，Ex/Em 490/525 nm）和超氧化物检测试剂（橙色，Ex/Em 550/620 nm）。试剂盒内还包括阳性对照，绿脓菌素和L-精氨酸，它们分别是ROS和NO产生的常见诱导剂；阴性对照N-乙酰-L-半胱氨酸和c-PTIO则分别是ROS和NO的常见清除剂。通过将三种检测探针与一组特异性抑制剂、激活剂相结合，能够区分超氧化物、一氧化氮和过氧亚硝酸盐。
产品特点
● 直接监测活细胞中的活性氧和氮化物（ROS/RNS）
● 可区分超氧化物、一氧化氮和过氧亚硝酸盐
● 高灵敏度、特异性和准确性，可用于活细胞研究
● 与组织培养基的主要成分（酚红、FBS和BSA）兼容
● 试剂盒内包含整套试剂，包括ROS/RNS诱导剂和清除剂
实验示例
图1. 氧化应激检测试剂（绿色）、超氧化物检测试剂（蓝色）和NO检测试剂（红色）的激发和发射光谱。
图2. 使用ROS/RNS检测试剂盒演示HeLa细胞中过氧亚硝酸盐和NO生成的代表性实验。分析表明，由于NO与超氧化物的反应，L-精氨酸和绿脓菌素的联合处理产生过氧亚硝酸盐（绿色荧光），但很少产生NO（红色荧光）。
产品信息
|
产品货号 |
ENZ-51001-200 |
|
产品名称 |
ROS-ID® ROS/RNS detection kit / 欣博盛生物 |
|
别名 |
Reactive oxygen species / Reactive nitrogen species |
|
规格 |
1*1Kit |
|
长期保存 |
-80°C |
|
试剂盒组分 |
NO Detection Reagent (Red), 60µl Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles NO Inducer (L-Arginine), 100 µl ROS Inducer (Pyocyanin), 1 µmole NO Scavenger (c-PTIO), 400 nmoles ROS Inhibitor (N-acetyl-L-cysteine), 2x10 mg 10X Wash Buffer, 15 ml |
|
应用 |
Fluorescence microscopy |
部分产品引用文献
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2. Dihydroartemisinin-transferrin adducts enhance TRAIL-induced apoptosis in triple-negative breast cancer in a P53-independent and ROS-dependent manner: X. Zhou, et al.; Front. Oncol. 11, 789336 (2022)
3. Intermittent exposure of cultured endothelial cells to physiologically relevant fructose concentrations has a profound impact on nitric oxide production and bioenergetics: M.L. Fiorello, et al.; PLoS One 17, e0267675 (2022)
4. S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease: B. Katunaric, et al.; Hypertension 79, 2250 (2022)
5. Extra-mitochondrial citrate synthase initiates calcium oscillation and suppresses age-dependent sperm dysfunction: W. Kang, et al.; Lab. Invest. 100, 583 (2020)
6. Liposome-encapsulated bacillus calmette-guérin cell wall skeleton enhances antitumor efficiency for bladder cancer in vitro and in vivo via induction of amp-activated protein kinase: Y.M. Whang, et al.; Cancers (Basel) 12, 3679 (2020)
7. Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells: A. Sanap, et al.; Chronic Dis. Transl. Med. 7, 47 (2020)
8. Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression: Q. Wu, et al.; Phytomedicine 57, 129 (2019)
9. Low intensity ultrasound reduces high glucose reduced nitric oxide generation in retinal pigment epithelial cells: M.B. Karmacharya, et al.; Ultrasound Med. Biol. 44, 647 (2018)
10. HEK-293 secretome attenuates kainic acid neurotoxicity through insulin like growth factor-phosphatidylinositol-3-kinases pathway and by temporal regulation of antioxidant defense machineries: C. Venugopal, et al.; Neurotoxicology 69, 189 (2017)
11. Selective enhancing effect of metal ions on mutagenicity: N. Fujii, et al.; Genes Environ. 38, 21 (2016)
12. Cell-to-cell diffusion of glucose in the mammalian heart is disrupted by high glucose. Implications for the diabetic heart: W.C. De Mello ; Exp. Cell Res. 334, 239 (2015)
13. Increased Proinflammatory Cytokine Production and Decreased Cholesterol Efflux Due to Downregulation of ABCG1 in Macrophages Exposed to Indoxyl Sulfate: K. Matsuo, et al. ; Toxins (Basel) 7, 3155 (2015)
14. Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts: G.E. Malpass, et al.; Toxicol. Appl. Pharmacol. 279, 211 (2014)
15. Enterococcus faecalis infection causes inflammation, intracellular oxphos-independent ROS production, and DNA damage in human gastric cancer cells: J.A. Strickertsson, et al.; PLoS One 8, e63147 (2013)
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