组胺是由氨基酸组氨酸合成的内源性短效生物胺，广泛分布于全身。组胺是速发型过敏反应的一种重要介质。正常情况下，人体内仅存在少量的组胺。当一种物质进入人体时（比如食物、化学品、昆虫叮咬、植物油脂），可能会引发过敏反应。作为对该分子的反应，身体就会释放更大量的组胺并开始免疫反应。身体通过产生肾上腺素（肾上腺素）来平衡组胺的释放，这可以帮助调节组胺的影响。由于组胺在免疫应答中的强有力作用，组胺在体内存在的时间很短暂，只能在释放后几分钟内测定。组胺也是一类小分子神经递质，这类物质包括5-羟色胺，肾上腺素和多巴胺等。此外，组胺在胃酸分泌中起作用，有助于诱导胃酸的产生。
ENZO Histamine ELISA kit是一种比色竞争法免疫测定试剂盒，可在2.5小时内得到检测结果。
● 高灵敏度，可检测低至0.03 ng / mL Histamine
● 独特的检测方法，不需要额外的酰化步骤
● 检测范围宽，适用于各种类型样品
● 高通量，可检测多达40个样品（带复孔），2.5可获得结果
产品信息
|
产品货号 |
ENZ-KIT140A-0001 |
|
产品名称 |
Histamine ELISA kit（组胺ELISA试剂盒） |
|
规格 |
96 wells |
|
灵敏度 |
0.068 pg/ml |
|
检测范围 |
0.098-25 pg/ml |
|
检测时间 |
2.5 hours |
|
样本种属 |
Human, Mouse, Rat |
|
样本类型 |
tissue culture media, serum, plasma and urine in human, mouse, and rat (serum only) samples |
|
保存温度 |
Store all components at +4°C, except conjugate, antibody, and tracer, which are stored at -20°C |
|
试剂盒组分 |
Microtiter Plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Tracer, Conjugate, Substrate, and Stop Solution |
部分产品文献引用
1. The allergy mediator histamine confers resistanceto immunotherapy in cancer patients via activationof the macrophage histamine receptor H1: H. Li, et al.; Cancer Cell 40, 36 (2022)
2. Angelica gigas extract ameliorates allergic rhinitis in an ovalbumin-induced mouse model by inhibiting Th2 cell activation: K.Y. Ji, et al.; Phytomedicine 93, 153789 (2021)
3. Anti-allergic effects of Asarum heterotropoides on an ovalbumin-induced allergic rhinitis murine model: S. Choi, et al.; Biomed. Pharmacother. 141, 111944 (2021)
4. Antibacterial and anti-inflammatory effects of Lactobacillus reuteri in its biofilm state contribute to its beneficial effects in a rat model of experimental necrotizing enterocolitis: R.D. Shelby, et al.; J. Pediatr. Surg. 22, 3468 (2021)
5. Rab44 isoforms similarly promote lysosomal exocytosis, but exhibit differential localization in mast cells: T. Kadowaki, et al.; FEBS Open Bio. 11, 1165 (2021), Application(s): Cell culture supernatant, BMMCs and RBL‐2H3
6. Histamine-mediated autocrine signaling in mesenteric perilymphatic mast cells: S. Pal, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 318, R590 (2020), Application(s): Histamine concentration in lysates from rat mesenteric perilymphatic tissue
7. A connective tissue mast-cell-specific receptor detects bacterial quorum-sensing molecules and mediates antibacterial immunity: P. Pundir, et al.; Cell. Host. Microb. 26, 114 (2019), Application(s): Analysis of culture supernatants
8. A Forward Chemical Genetic Screen Reveals Gut Microbiota Metabolites That Modulate Host Physiology: H. Chen, et al.; Cell 177, 1217 (2019), Application(s): Quantification of histamine production by 144 isolates of human gut bacteria
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