MAP2K2 (Human) Recombinant Protein-蛋白质/抗原/多肽-试剂-生物在线
亚诺法生技股份有限公司（Abnova)
MAP2K2 (Human) Recombinant Protein

MAP2K2 (Human) Recombinant Protein

商家询价

产品名称： MAP2K2 (Human) Recombinant Protein

英文名称： MAP2K2 (Human) Recombinant Protein

产品编号： P5583

产品价格： 0

产品产地： 台湾

品牌商标： Abnova

更新时间： null

使用范围： null

亚诺法生技股份有限公司（Abnova)
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  • Specification
  • Product Description:
  • Human MAP2K2 (NP_109587.1, 1 a.a. - 400 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
  • Theoretical MW (kDa):
  • 72
  • Form:
  • Liquid
  • Preparation Method:
  • Baculovirus infected insect cell (Sf21) expression system
  • Purification:
  • Glutathione sepharose chromatography
  • Purity:
  • 96 % by SDS-PAGE/CBB staining
  • Activity:
  • The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG (or HRP-labeled anti-mouse IgG). Substrate: Erk2 [inactive mutant]. ATP: 100 uM.
  • Storage Buffer:
  • In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Quality Control Testing:
  • Loading 1 ug protein in SDS-PAGE

    QC Testing of P5583
  • Note:
  • Result of activity analysis

  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 5605
  • Gene Name:
  • MAP2K2
  • Gene Alias:
  • FLJ26075,MAPKK2,MEK2,MKK2,PRKMK2
  • Gene Description:
  • mitogen-activated protein kinase kinase 2
  • Gene Summary:
  • The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq
  • Other Designations:
  • ERK activator kinase 2,MAP kinase kinase 2,MAPK/ERK kinase 2,dual specificity mitogen-activated protein kinase kinase 2,mitogen-activated protein kinase kinase 2, p45

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