Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)
产品名称： Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)
英文名称： Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)
产品编号： 93111ES25
产品价格： 0
产品产地： Yeasen
品牌商标： Yeasen
更新时间： 2024-12-10T13:22:33
使用范围： null
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产品信息
产品名称
产品编号
规格
价格（元）
Recombinant Human B7-H3(4Ig)/B7-H3b (hFc Tag)
93111ES25
25 μg
1185
93111ES60
100 μg
3185
93111ES76
500 μg
12235
93111ES80
1 mg
21825
产品描述
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V-like and Ig C-like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V-type and one C-type Ig domain, a transmembrane segment and a short cytoplasmic tail. Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C-type) and shows a ubiquituous expression pattern. It would appear that the human 4Ig form is the principal, if not the only form of B7-H3. Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V-type and C‑type, and apparently are the consequence of a V-C type tandem duplication. B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells.
产品性质
别名
B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Uniprot No.
Q5ZPR3.1
表达区间及表达系统
Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Gly27-Thr461.
分子量
Approximately 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
纯度
> 95% as determined by SDS-PAGE and HPLC.
活性
ELISA Data: Immobilized Human B7-H3 (4Ig) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA.
内毒素
< 1.0 EU per 1μg of the protein by the LAL method.
制剂
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
复溶
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220804
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