Synonyms

Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, Pluripoietin-α

Uniprot No.

P01587

Source

Recombinant Mouse GM-CSF Protein is expressed from Yeast with N-HIS. It contains Ala18-Lys141.

Molecular Weight

The recombinant mouse GM-CSF comprises 124 amino acids and has a predicted molecular mass of 14.1 kDa.

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

<1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4,with 1%BSA.

Reconstitution

1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL.

2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

3. Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum.